About me

I am a Postdoctoral Research Scientist at Abbvie Inc. I completed by PhD in Medicinal Chemistry and Cancer Pharmacology at Purdue advised by Michael Wendt.

I am mainly interested in Antibody Drug Conjugates and Cancer Immunotherapy. Overall my research combines genetics, cell biology and immunology to unravel the molecular mechanisms in breast cancer metastasis. I’ve gained 8 years of hands-on expertise in biochemical, molecular and cellular biological techniques and have solid grounding in statistical principles. I’m constantly adding to my skills and am currently adept at

  • Cell line engineering using lentiviral/retroviral transductions
  • protein expression, purification and characterization
  • iPSc/primary/mammalian cell culture, PCR, cloning, FACS
  • AKTA FPLC, LC-MS, ELISA, CRISPR
  • Office, Prism, Python, PyMol, JMP, Flowjo

My research so far has generated 6 first author and 2 co-author peer reviewed journal articles. I’ve had opportunity to lead research on 7 individual and 5 collaborative NCI funded projects.

Publications

drawingSpleen Tyrosine Kinase–Mediated Autophagy Is Required for Epithelial–Mesenchymal Plasticity and Metastasis in Breast Cancer
Aparna Shinde, Shana D. Hardy, Dongwook Kim, Saeed Salehin Akhand, Mohit Kumar Jolly, Wen-Hung Wang, Joshua C. Anderson, Ryan B. Khodadadi, Wells S. Brown, Jason T. George, Sheng Liu, Jun Wan, Herbert Levine, Christopher D. Willey, Casey J. Krusemark, Robert L. Geahlen and Michael K. Wendt
Cancer Research 2019 [Link] [Commentary] [Purdue News]
Published as Cover of April 2019 Issue
drawingAutocrine fibronectin inhibits breast cancer metastasis
Aparna Shinde, S. Libring, A. Alposy, A. Abdullah, J.A. Schaber, L. Solorio and M. K. Wendt
Molecular Cancer Research 2018 [pdf] [Supp. info] [video1] [video2] [video3] [video4] [video5]
Published as Cover of October 2018 Issue
drawingPyruvate carboxylase drives pulmonary tropism of breast cancer
Aparna Shinde, T. Wilmanski, H. Chen, D. Teegarden and M. K. Wendt
Breast Cancer Research 2018 [pdf]
drawingToxicity mechanism in fetal lung fibroblast cells for multi-walled carbon nanotubes defined by chemical impurities and dispersibility
Aparna Shinde and C. Tsai
Toxicology Research 2016 [pdf]
Top 30-Most Accessed Articles for 2016 in Toxicology Research
drawingPeptide internalization enabled by folding: triple helical cell-penetrating peptides
Aparna Shinde, K. Feher, C. Hu and K. Slowinska
Journal of Peptide Science 2015 [pdf]
drawingRegulation of epithelial-mesenchymal transition and metastasis by TGF-β, P-bodies, and autophagy
S.Hardy, Aparna Shinde, W. Wang, M. Wendt, and R. Geahlen
Oncotarget 2017 [pdf]
drawingInhibition of pyruvate carboxylase by 1α,25-dihydroxyvitamin D promotes oxidative stress in early breast cancer progression
T. Wilmanski, X. Zhou, W. Zheng, Aparna Shinde, S.S. Donkin, M. Wendt, J. R. Burgess, and D. Teegarden
Cancer Letters 2017 [pdf]

Talks

  • 2018: Oral Presentation at Annual Amelia Project Conference, “Fibronectin mediates epithelial-mesenchymal transition in metastatic breast cancer”
  • 2015: Oral presentation at American Industrial Hygiene Association (AIHA) - Chicago chapter, “Particle characterization factors responsible for malignant transformation of lung cells associated with exposure to carbon nanoparticles”